Journal: eLife

Article Title: Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury

doi: 10.7554/eLife.43882

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , Fluidigm Single-Cell Auto Prep IFC chip (5–10 um) , Fluidigm , 100–5759 , .

Techniques: Multiplex Assay, Sample Prep, Software

Journal: Leukemia

Article Title: Single-cell sequencing reveals the origin and the order of mutation acquisition in T-cell acute lymphoblastic leukemia

doi: 10.1038/s41375-018-0127-8

Figure Lengend Snippet: Multiple mutations can be present in multipotent progenitor cells. a Bar chart of the absolute numbers of single CD34 + CD38 - multipotent progenitor cells isolated per patient and the percentage of cells accepted for analysis after quality control. b Heatmaps of the variations in single CD34 + CD38 - multipotent progenitor cells isolated from patient X09, XB37, XB41 or XB47 taken at diagnosis (i) and at remission (iii). Sanger sequencing was performed on bulk DNA extracted from 2000 to 5000 myeloid progenitor cells sorted from the diagnostic samples (ii) to confirm the presence of the mutations found in the multipotent progenitor cells at diagnosis. Deletions and fusion genes were not evaluated in the bulk myeloid progenitor DNA to prevent false-positive results caused by few contaminating leukemic cells, and are therefore colored white in the graph. The order of both the cells and the variations is based on hierarchical clustering with the Jaccard distance as metric. *These variations were initially considered somatic mutations, based on the WGS results of the remission sample. However, we could confirm the presence of these SNPs with PCR on the bulk remission samples

Article Snippet: Cells were washed and prepared for single-cell isolation on a small C1 DNA sequencing chip (IFC, 5–10 μm, Fluidigm).

Techniques: Isolation, Control, Biomarker Discovery, Sequencing, Diagnostic Assay